PA5-19963

antibody from Invitrogen Antibodies

Targeting: CASP12

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-19963 - Provider product page
Provider: Invitrogen Antibodies
Product name: Caspase 12 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control is mouse brain tissue lysate. PA5-19963 can be used with blocking peptide PEP-0088.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

CASP12 protein structure - PA5-19963 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. DZX regulates the expression of CHOP, GRP78, caspase-12 and caspase-3 in OGD-treated hippocampal cells. (A) Immunohistochemical staining demonstrated the distribution of GRP78 and CHOP in hippocampal cells exposed to OGD. (B) Western blot analysis was used to detect the expression of GRP78 and CHOP in hippocampal cells exposed to OGD. (C) Protein expression levels of caspase-12 and caspase-3 in hippocampal cells exposed to OGD were detected using western blotting. Data are expressed as the mean +- standard deviation. *P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. Pretreatment with DZX modulates the protein expression of Bcl-2, caspase-12, caspase-3, Bax, CHOP and GRP78 in rats subjected to I/R. Western blot analysis was used to detect the protein expression levels of Bcl-2, caspase-12, caspase-3, Bax, CHOP and GRP78 in hippocampal cells isolated from I/R-treated rats. Rats were treated with DZX with or without 5-HD and subjected to cerebral ischemia for 2 h followed by 12 h of reperfusion. Sham rats were not subjected to cerebral ischemia. DZX, diazoxide; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X protein; CHOP, C/EBP homologous protein; GRP78, 78 kDa glucose-regulated protein; I/R, ischemia/reperfusion; 5-HD, 5-hydroxydecanoate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 5 Inhibition of autophagy abolishes PNS protection on TG-induced ER stress response and associated apoptosis. (A) SiNC or siATG7 transfected H9c2 cells, either untreated (CN group) or pretreated with 40 mug/ml PNS for 12 h before addition of 1 muM TG (TG group or PNS plus TG group) for 12 h were immunofluorescenced with the primary anti-calnexin antibody and imaged by a laser scanning confocal microscopy. Scale bar: 30 mum; in box: 10 mum. (B) SiNC or siATG7 transfected H9c2 cells, treated as in A, were immunoblotted with the antibodies to BiP, CHOP, Cleaved Caspase-3 and Caspase-12 as well as beta -actin. Bands were quantified relative to beta -actin by densitometry (Mean +- SEM; ** p < 0.01 relative to CN group, or indicated group). (C) SiNC or siATG7 transfected H9c2 cells, treated as in A, were stained with TUNEL and imaged by a laser scanning confocal microscopy. Scale bar: 200 mum. Bar graph shows the percentage of TUNEL positive cells (Mean +- SEM; 200-250 cells; ** p < 0.01 relative to CN group, or indicated group).