AF7724

antibody from R&D Systems

Targeting: AMBP EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7724

Provider

R&D Systems

Product name

Human alpha 1-Microglobulin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human alpha 1-Microglobulin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat alpha 1-Microglobulin is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P02760


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human alpha 1-Microglobulin by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human alpha 1-Microglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7724) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha 1-Microglobulin at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.