Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- BAF1014 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Cathepsin D Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Cathepsin D in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse Cathepsin D is observed, and less than 1% cross-reactivity with recombinant human (rh) Cathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin L, and rhCathepsin X/Z/P is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cathepsin D in Human Lung. Cathepsin D was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human Cathepsin D Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1014) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.