Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-72182 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cathepsin D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.35 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTSD in HepG2, MCF-7, A431 cell line lysate using 35 µg of protein per lane. Samples were probed with CTSD polyclonal antibody (Product # PA5-72182) with a dilution of 1:1000, and a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CTSD in HepG2 cells. Cells were incubated with CTSD polyclonal antibody (Product # PA5-72182) using a dilution of 1:25. Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) with a dilution of 1:400 was used as the secondary antibody. Cytoplasmic actin was detected with Alexa Fluor 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CTSD in paraffin-embedded human liver tissue. Samples were incubated with CTSD polyclonal antibody (Product # PA5-72182) using a dilution of 1:25. A peroxidase-conjugated goat anti-rabbit IgG secondary antibody with a dilution of 1:400 was used followed by DAB staining.