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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00001509-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001509-M01, RRID:AB_537443
- Product name
- Mouse Monoclonal Cathepsin D Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CTSD - cathepsin D (lysosomal aspartyl protease)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Characterization of the human gastric fluid proteome reveals distinct pH-dependent protein profiles: implications for biomarker studies.
Kam SY, Hennessy T, Chua SC, Gan CS, Philp R, Hon KK, Lai L, Chan WH, Ong HS, Wong WK, Lim KH, Ling KL, Tan HS, Tan MM, Ho M, Kon OL
Journal of proteome research 2011 Oct 7;10(10):4535-46
Journal of proteome research 2011 Oct 7;10(10):4535-46
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cathepsin D Antibody (3F12-1B9) [H00001509-M01] - Analysis of CTSD expression in transfected 293T cell line by CTSD monoclonal antibody (M01), clone 3F12-1B9.Lane 1: CTSD transfected lysate (Predicted MW: 44.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Cathepsin D Antibody (3F12-1B9) [H00001509-M01] - Detection limit for recombinant GST tagged CTSD is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cathepsin D Antibody (3F12-1B9) [H00001509-M01] - Analysis of CTSD transfected lysate using anti-CTSD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CTSD MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cathepsin D Antibody (3F12-1B9) [H00001509-M01] - Analysis of monoclonal antibody to CTSD on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
