Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-17353 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cathepsin D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 16 µg/mL
- Storage
- -20°C
Submitted references Regulation of endo-lysosomal pathway and autophagic flux by broad-spectrum antipathogen inhibitor ABMA.
Wu Y, Boulogne C, Carle S, Podinovskaia M, Barth H, Spang A, Cintrat JC, Gillet D, Barbier J
The FEBS journal 2020 Aug;287(15):3184-3199
The FEBS journal 2020 Aug;287(15):3184-3199
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) MCF7 (Lane 2), Panc-1 (Lane 3) and U-937 (Lane 4). The blot was probed with anti-Cathepsin D Rabbit Polyclonal Antibody (Product # PA5-17353, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to Cathepsin D was detected across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cathepsin D in extracts from SNB19 and MCF-7 cell lines using Cathepsin D polyclonal antibody (Product # PA5-17353).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Cathepsin D was achieved by transfecting MCF7 with Cathepsin D specific siRNAs (Silencer® select Product # s137). Western blot analysis (Fig. a) was performed using whole cell extracts from the MCF7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Cathepsin D Polyclonal Antibody (Product # PA5-17353, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Complete loss of signal upon siRNA mediated knock down confirms that antibody is specific to Cathepsin D.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cathepsin D in paraffin-embedded human lung carcinoma using a Cathepsin D polyclonal antibody (Product # PA5-17353).