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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Immunohistochemistry [1]
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- Product number
- BAF1014 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cathepsin D Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Cathepsin D in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse Cathepsin D is observed, and less than 1% cross-reactivity with recombinant human (rh) Cathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin L, and rhCathepsin X/Z/P is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P07339- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cathepsin D in Human Lung. Cathepsin D was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human Cathepsin D Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1014) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
