MAB4926

antibody from Novus Biologicals

Targeting: PON1 ESA, PON

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4926

Provider

Novus Biologicals

Product name

Rat Monoclonal PON1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects endogenous human and mouse PON1 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON2 or rhPON3.

Reactivity

Human, Mouse

Host

Rat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse PON1 by Western Blot. Western blot shows lysates of mouse liver tissue, human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse PON1 Monoclonal Antibody (Catalog # MAB4926) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.