PA5-28997

antibody from Invitrogen Antibodies

Targeting: PON1 ESA, PON

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28997

Provider

Invitrogen Antibodies

Product name

PON1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: human plasma, human liver, HepG2. Predicted reactivity: Mouse (82%), Rat (81%), Pig (84%), Rabbit (85%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Spp24 is associated with endocytic signalling, lipid metabolism, and discrimination of tissue integrity for 'leaky-gut' in inflammatory bowel disease.

Wasinger VC, Lu K, Yau YY, Nash J, Lee J, Chang J, Paramsothy S, Kaakoush NO, Mitchell HM, Leong RWL
Scientific reports 2020 Jul 31;10(1):12932

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Expression levels of paraoxonase-1 in aortic valve tissue are associated with the progression of calcific aortic valve stenosis.

Song J, Zheng Q, Ma X, Zhang Q, Xu Z, Zou C, Wang Z
Journal of thoracic disease 2019 Jul;11(7):2890-2898

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Dysregulated Lipid Transport Proteins Correlate With Pathogenesis and Outcome in Severe Alcoholic Hepatitis.

Maras JS, Das S, Bhat A, Kumar Vyas A, Yadav G, Chaudhary S, Sukriti S, Gupta AC, Bihari C, Mahiwall R, Sarin SK
Hepatology communications 2019 Dec;3(12):1598-1625

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PON1 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PON1 polyclonal antibody (Product # PA5-28997) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PON1 in Human tissue extracts (30 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with PON1 Polyclonal antibody (Product # PA5-28997) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PON1 Polyclonal Antibody detects PON1 protein by western blot analysis. Human tissue extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PON1 Polyclonal Antibody (Product # PA5-28997) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PON1 Polyclonal Antibody detects PON1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PON1 Polyclonal Antibody (Product # PA5-28997) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Quantitative proteomic analysis identifies significantly down-regulated lipid transporter proteins in SAH. (A) Partial list of proteins differentially regulated (>1.5-fold; P < 0.05) between SAH, AC, HC, and its GO analysis, which documents enrichment of lipid transporters in SAH. (B) KEGG enrichment analysis of the up-regulated (n = 32) and down-regulated (n = 176) proteins in patients with SAH represents significant ( P < 0.05) enrichment. (C) Plasma level of PON1 was down-regulated in patients with SAH (median, 23.2; range, 2.3-139.4) compared to those with AC (median, 44.7; range, 25.8-131) and HC (median, 139.5; range, 46.6-196.0) ( P < 0.05). (D) Plasma level of apolipoproteins (APOB, APOE, APOA1, APOA2, APOC1, APOC3) in SAH compared to AC and HC ( P < 0.05 is significant). (E) IHC showing expression of PON1, LDL, CD36, SRA1, ABCA1, and ABCG1 in SAH (n = 5) and AC (n = 5). For all IHC analyses, relative quantization of positively stained cells are expressed as mean number of positive cells/10 high-power field (40x) and * P < 0.05. Abbreviations: COA, coenzyme A; HBG1, hemoglobin subunit gamma 1; KEGG, Kyoto Encyclopedia of Genes and Genomes; LBP, lipopolysaccharide binding protein; LPS, lipopolysaccharide; TLR4, toll-like receptor 4;