Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AF3476 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal RBP4/Retinol-Binding Protein 4 Antibody
- Antibody type
- Polyclonal
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse RBP4/Retinol-Binding Protein 4 by Western Blot. Western blot shows lysate of Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse RBP4/Retinol-Binding Protein 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3476) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.