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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- MAB34761 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal RBP4/Retinol-Binding Protein 4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse RBP4/Retinol-Binding Protein 4 in direct ELISA and Western Blot. In Western blots, detects human RBP4/Retinol-Binding Protein 4.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation.
Yentrapalli R, Merl-Pham J, Azimzadeh O, Mutschelknaus L, Peters C, Hauck SM, Atkinson MJ, Tapio S, Moertl S
International journal of radiation biology 2017 Jun;93(6):569-580
International journal of radiation biology 2017 Jun;93(6):569-580
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse RBP4/Retinol-Binding Protein 4 by Western Blot. Western blot shows lysates of mouse serum. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human RBP4/Retinol-Binding Protein 4 by Western Blot. Western blot shows lysates of human liver tissue and human serum. PVDF membrane was probed with 0.05 µg/mL of Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of RBP4/Retinol-Binding Protein 4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34761, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
