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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [2]
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- Product number
- NBP1-05426 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-05426, RRID:AB_1556456
- Product name
- Mouse Monoclonal RBP4/Retinol-Binding Protein 4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Human plasma RBP4. All MAbs recognize free RBP4 and RBP4 in complex with transthyretin. All MAbs react with apo- as well as holo-RBP4.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunodetection of RBP4 in Western blotting after SDSelectrophoresis in reducing conditions by MAb RB42 (Cat. NBP1-05426) (lane 1), RB45 (Cat. NBP1-105431) (lane 2), RB48 (Cat. NBP1-105430) (lane 3) and RB51 (Cat.NBP1-105428) (lane 4).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunoreactivity of native free and native complexed RBP4 after several freeze-thaw cycles measured with the assay RB48 - RB42. Capture antibody: RB48 (Cat. NBP1-105430) (1 ug/well) Detection antibody: RB42 (Cat NBP1-05426) labeled with stable Eu3+ chelate (0.2ug/well) Antigen: Native isolated RBP4.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunodetection of purified endogenous RBP4 in sandwich immunoassay by RB48-RB42 MAb assay in presence of 5 mM EDTA (blue line) or in absence of EDTA (pink line). Capture antibody: RB48 (Cat NBP1-105430) (1 ug/well) Detection antibody: RB42 (Cat NBP1-05426) labeled with stable Eu3+ chelate (0.2ug/well) Antigen: Native isolated RBP4
