H00009063-M01

antibody from Novus Biologicals

Targeting: PIAS2 ARIP3, miz, PIASX-ALPHA, PIASX-BETA, ZMIZ4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

H00009063-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00009063-M01, RRID:AB_547028

Product name

Mouse Monoclonal PIAS2 Antibody

Antibody type

Monoclonal

Description

IgG purified. PIAS2 - protein inhibitor of activated STAT, 2

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of PIAS2 over-expressed 293 cell line, cotransfected with PIAS2 Validated Chimera RNAi ( Cat # H00009063-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIAS2 monoclonal antibody (M01), clone 1F7 (Cat # H00009063-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: PIAS2 Antibody (1F7) [H00009063-M01] - Detection limit for recombinant GST tagged PIAS2 is approximately 0.3ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of monoclonal antibody to PIAS2 on HeLa cell . Antibody concentration 10 ug/ml.