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LearnMA5-26974
antibody from Invitrogen Antibodies
Targeting: PIAS2
ARIP3, miz, PIASX-ALPHA, PIASX-BETA, ZMIZ4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
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- Product number
- MA5-26974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIAS2 Monoclonal Antibody (OTI5B4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI5B4
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIAS2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PIAS2 (Product # MA5-26974) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIAS2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PIAS2 (Product # MA5-26974) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIAS2 in Jurkat, HepG2 and K562 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with PIAS2 (Product # MA5-26974) monoclonal antibody at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PIAS2 Monoclonal Antibody (OTI5B4) (Product # MA5-26974) and a 63-68kDa band corresponding to E3 SUMO-protein ligase PIAS2 was observed across cell lines and tissue extract tested except Mouse Prostate, Mouse Spleen and Mouse Brain which is reported to be low. Fresh nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), DU 145 (Lane 2), LNCaP (Lane 3), Jurkat (Lane 4), K-562 (Lane 5), PC-3 (Lane 6), and tissue extracts (30 µg lysate) of Mouse Prostate (Lane 7), Mouse Spleen (Lane 8), Mouse Brain (Lane 9), and Mouse Testis (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
