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LearnPA5-30297
antibody from Invitrogen Antibodies
Targeting: PIAS2
ARIP3, miz, PIASX-ALPHA, PIASX-BETA, ZMIZ4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-30297 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIAS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIAS2 using 30 µg of A) Jurkat (B) Raji (C) K562 (D) THP-1 (E) HL-60 and F) NCI-H929 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PIAS2 polyclonal antibody (Product # PA5-30297) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PIAS2 Polyclonal Antibody (Product # PA5-30297). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 7.5% SDS PAGE. PIAS2 Polyclonal Antibody (Product # PA5-30297) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PIAS2 Polyclonal Antibody (Product # PA5-30297). Sample (30 µg of whole cell lysate). Lane A: Jurkat. Lane B: Raji. Lane C: K562. Lane D: THP-1. Lane E: HL-60. Lane F: NCI-H929. 7.5% SDS PAGE. PIAS2 Polyclonal Antibody (Product # PA5-30297) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PIAS2 (Product # PA5-30297) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
