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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- NBP2-22453 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal ARNT/HIF-1 beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100uL
- Concentration
- 0.6 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody [NBP2-22453] - Analysis of aryl hydrocarbon receptor (green) in NRK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with an aryl hydrocarbon receptor antibody at a dilution of 20ug/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye.
