14105-1-AP

antibody from Invitrogen Antibodies

Targeting: ARNT bHLHe2, HIF-1beta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14105-1-AP

Provider

Invitrogen Antibodies

Product name

ARNT/HIF1B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAATTANPE MTSDVPSLGP AIASGNSGPG IQGGGAIVQR AIKRRPGLDF DDDGEGNSKF LRCDDDQMSN DKERFARSDD EQSSADKERL ARENHSEIER RRRNKMTAYI TELSDMVPTC SALARKPDKL TILRMAVSHM KSLRGTGNTS TDGSYKPSFL TDQELKHLIL EAADGFLFIV SCETGRVVYV SDSVTPVLNQ QQSEWFGSTL YDQVHPDDVD KLREQLSTSE NALTGRILDL KTGTVKKEGQ QSSMRMCMGS RRSFICRMRC GSSSVDPVSV NRLSFVRNRC RNGLGSVKDG EPHFVVVHCT GYIKAWPPAG VSLPDDDPEA GQGSKFCLVA IGRLQVTSS (1-348 aa encoded by BC060838 )

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT,HIF1B antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT,HIF1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of ARNT,HIF1B antibody (14105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT,HIF1B transfected HeLa cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 14105-1-AP (ARNT,HIF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 14105-1-AP (ARNT,HIF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT,HIF1B Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT,HIF1B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 14105-1-AP ( ARNT,HIF1B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ARNT,HIF1B (IP:14105-1-AP, 4ug; Detection:14105-1-AP 1:1000) with HepG2 cells lysate 3600ug.