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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA3-16508 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIF-1 beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- In Western blot, background may increase with whole cell extract preparations. Lower dilutions may be needed for whole cell extract preps. Suggested positive control: MCF-7 human nuclear extracts, MCF-7 whole cell lysate, antigen standard for ARNT (transient overexpression lysate).
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20°C or -80°C if preferred
Submitted references A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A.
Colombo F, Falvella FS, Galvan A, Frullanti E, Kunitoh H, Ushijima T, Dragani TA
Molecular cancer 2011 Jul 7;10:81
Molecular cancer 2011 Jul 7;10:81
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HIF-1 beta in ARNT/HIF-1 beta antibody in MCF7 whole cell lysate. Samples were incubated in HIF-1 beta polyclonal antibody (Product # PA3-16508). Theoretical molecular weight is 86.6 kDa. Observed molecular weight ~95 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HIF-1 beta in 0.5 mg/mL Hypoxic HeLa lysate. Samples were incubated in HIF-1 beta polyclonal (Product # PA3-16508). This experiment was performed under reducing conditions using the 12-230 kDa separation system. Theoretical molecular weight is 86.6 kDa. Observed molecular weight ~106 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HIF-1 beta in human skin epidermis. Samples were incubated in HIF-1 beta polyclonal antibody (Product # PA3-16508).
