MA5-15810

antibody from Invitrogen Antibodies

Targeting: OLIG2 BHLHB1, bHLHe19, OLIGO2, PRKCBP2, RACK17

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15810

Provider

Invitrogen Antibodies

Product name

OLIG2 Monoclonal Antibody (1G11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15810 targets OLIG2 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15810 immunogen is purified recombinant fragment of human OLIG2 expressed in E. Coli. MA5-15810 detects OLIG2 which has a predicted molecular weight of approximately 32kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1G11

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Tissue Niche Miniature of Glioblastoma Patient Treated with Nano-Awakeners to Induce Suicide of Cancer Stem Cells.

Yoon SJ, Baek S, Yu SE, Jo E, Lee D, Shim JK, Choi RJ, Park J, Moon JH, Kim EH, Chang JH, Lee JB, Park JS, Sung HJ, Kang SG
Advanced healthcare materials 2022 Nov;11(21):e2201586

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In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function.

Sharma Y, Saha S, Joseph A, Krishnan H, Raghu P
Wellcome open research 2020;5:16

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Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex.

Wong FK, Fei JF, Mora-Bermúdez F, Taverna E, Haffner C, Fu J, Anastassiadis K, Stewart AF, Huttner WB
PLoS biology 2015 Aug;13(8):e1002217

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OLIG2 using OLIG2 monoclonal antibody (Product # MA5-15810) in HEK293 (1) and OLIG2 (AA: 1-122) human IgG Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of U251 cells using OLIG2 monoclonal antibody (Product # MA5-15810) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of U251 cells using OLIG2 monoclonal antibody (Product # MA5-15810) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of U251 cells using OLIG2 monoclonal antibody (Product # MA5-15810) (Green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded muscle tissues using OLIG2 monoclonal antibody (Product # MA5-15810) followed with DAB staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Expression of synaptic and glial markers during in-vitro differentiation. ( A ) to ( C ) Relative mRNA expression levels of synaptic markers SYN , SYP and PSD95 at stages of neuronal differentiation obtained from quantitative real time PCR (qRT PCR). The expression levels have been normalized with GAPDH as endogenous control and the values represented in the terms of 2 -Ct- Y-axis, mean +- SEM. ( D ) Presence of presynaptic protein Synapsin 1 (yellow) and ( E ) synaptic vesicle protein Synaptophysin (yellow) in MAP2 (red) positive neuronal cells. The nuclei of the cells were observed by staining with DAPI (blue). ( F ) to ( H ) qRT PCR results of relative mRNA expression in GABAergic neuronal marker GAD67 , and glutamatergic neuronal markers GLS and VGLUT1 respectively. ( I ) and ( J ) Relative mRNA expression levels of astrocyte and oligodendrocyte markers GFAP and OLIG2 respectively at different stages of neuronal differentiation, as obtained from qRT PCR. The expression levels of all the genes have been normalized with GAPDH as endogenous control and the values represented in the terms of 2 -Ct , mean +- SEM. One-way ANOVA was used for statistical analysis, and p < 0.05 was considered significant. ( K ) Immunofluorescence images (maximum intensity projections) showing expression of GFAP (magenta) and OLIG2 (green) at stages of neuronal differentiation from neural stem cells. The cells were stained with astrocyte marker GFAP and oligodendrocyte marker OL