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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA1-31853 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PCSK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-31853 has been successfully used in immunofluorescence and Western blot applications, where a band of approximately 82 kDa is detected.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.36 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PCSK2 using PCSK2 Polyclonal Antibody (Product # PA1-31853) on human U2OS cells. The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2. Immunofluorescence was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Polyethylene glycol tert-octylphenyl ether-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37° C. Nuclei were counterstained with Hoechst stain (blue).
