PA1-058

antibody from Invitrogen Antibodies

Targeting: PCSK2 NEC2, PC2, SPC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-058

Provider

Invitrogen Antibodies

Product name

PCSK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-058 detects prohormone convertase 2 (PC2) from human and mouse alpha pancreas cells. PA1-058 has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects an ~65 kDa protein representing PC2 from alpha TC 6 cell extract. This antibody is not recommended for Neuro-2a cells in immunfluorescent applications. The PA1-058 immunogen is a synthetic peptide corresponding to residues E(622) L D E A V E R S L Q S I L R K N(638) of mouse PC2. This sequence is completely conserved between mouse and rat protein. PA1-058 synthetic peptide (PEP-138) is available for use in neutralization and control experiments.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Revisiting Proinsulin Processing: Evidence That Human β-Cells Process Proinsulin With Prohormone Convertase (PC) 1/3 but Not PC2.

Ramzy A, Asadi A, Kieffer TJ
Diabetes 2020 Jul;69(7):1451-1462

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Chromogranin B regulates early-stage insulin granule trafficking from the Golgi in pancreatic islet β-cells.

Bearrows SC, Bauchle CJ, Becker M, Haldeman JM, Swaminathan S, Stephens SB
Journal of cell science 2019 Jul 1;132(13)

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Insulin-Deficient Mouse β-Cells Do Not Fully Mature but Can Be Remedied Through Insulin Replacement by Islet Transplantation.

Ramzy A, Mojibian M, Kieffer TJ
Endocrinology 2018 Jan 1;159(1):83-102

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Compensatory Islet Response to Insulin Resistance Revealed by Quantitative Proteomics.

El Ouaamari A, Zhou JY, Liew CW, Shirakawa J, Dirice E, Gedeon N, Kahraman S, De Jesus DF, Bhatt S, Kim JS, Clauss TR, Camp DG 2nd, Smith RD, Qian WJ, Kulkarni RN
Journal of proteome research 2015 Aug 7;14(8):3111-3122

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PC2 was performed by loading 25 µg of mouse cerebellum cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a PC2 polyclonal antibody (Product # PA1-058) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~65 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PC2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PC2 polyclonal antibody (Product # PA1-058) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PC2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PC2 polyclonal antibody (Product # PA1-058) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.