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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- NBP2-33229 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal MITF Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Mitf produces, by alternative splicing, 10 different transcripts, all with introns, putatively encoding 10 different protein isoforms. Therefore, observed molecular weights may vary depending on MITF isoforms expressed. Users are encouraged to utilize the peptide immunogen sequence to gain further information about the isoforms recognized by the antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MITF Antibody (21D1418) - Azide Free [NBP2-33229] - Analysis of HeLa lysate (35ug per lane, RIPA buffer) using MiTF antibody (NB100-56561) at 0.1ug/ml. Band detected at ~60kDa. (Expected MW of 58.2kDa according to NP_937802.1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MITF Antibody (21D1418) - Azide Free [NBP2-33229] - Analysis of A375 lysate using MITF antibody at 4 ug/ml.
