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Western blot
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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP1-88618 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88618, RRID:AB_11039748
- Product name
- Rabbit Polyclonal MITF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MITF antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references MITF and c-Jun antagonism interconnects melanoma dedifferentiation with pro-inflammatory cytokine responsiveness and myeloid cell recruitment.
Enhancer-targeted genome editing selectively blocks innate resistance to oncokinase inhibition.
Riesenberg S, Groetchen A, Siddaway R, Bald T, Reinhardt J, Smorra D, Kohlmeyer J, Renn M, Phung B, Aymans P, Schmidt T, Hornung V, Davidson I, Goding CR, Jönsson G, Landsberg J, Tüting T, Hölzel M
Nature communications 2015 Nov 4;6:8755
Nature communications 2015 Nov 4;6:8755
Enhancer-targeted genome editing selectively blocks innate resistance to oncokinase inhibition.
Webster DE, Barajas B, Bussat RT, Yan KJ, Neela PH, Flockhart RJ, Kovalski J, Zehnder A, Khavari PA
Genome research 2014 May;24(5):751-60
Genome research 2014 May;24(5):751-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MITF Antibody [NBP1-88618] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-MITF antibody. Corresponding MITF RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MITF Antibody [NBP1-88618] - Analysis in control (vector only transfected HEK293T lysate) and MITF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618] - Staining of human endometrium shows moderate nuclear positivity in cells in endometrial stroma.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618] - Staining of human skin shows moderate to strong nuclear positivity in melanocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MITF Antibody [NBP1-88618] - Staining of human pancreas shows no positivity as expected.
