600-401-A59

antibody from Rockland Immunochemicals, Inc.

Targeting: SMAD2 JV18-1, MADH2, MADR2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-A59

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-A59, RRID:AB_2286431

Product name

Anti-SMAD2 (RABBIT) Antibody - 600-401-A59

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration.

Chung S, Overstreet JM, Li Y, Wang Y, Niu A, Wang S, Fan X, Sasaki K, Jin GN, Khodo SN, Gewin L, Zhang MZ, Harris RC
JCI insight 2018 Nov 2;3(21)

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-Smad2 to detect over-expressed Smad2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of Smad2 in lysates of COS transfected with Smad2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGF?. Low levels of staining in control lanes correspond to detection of endogenous Smad2. Preincubation of the antibody with immunizing peptide (data not shown) completely blocks specific band staining. The blot presented is askew relative to the molecular weight markers. The expected MW for Smad2 is 52 kDa. The membrane was probed with the primary antibody at a 1:2500 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.

Validation comment

Western Blot