Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-A59S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-A59S, RRID:AB_2255104
- Product name
- Anti-SMAD2 (RABBIT) Antibody - 600-401-A59S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
Submitted references TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration.
Chung S, Overstreet JM, Li Y, Wang Y, Niu A, Wang S, Fan X, Sasaki K, Jin GN, Khodo SN, Gewin L, Zhang MZ, Harris RC
JCI insight 2018 Nov 2;3(21)
JCI insight 2018 Nov 2;3(21)
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-Smad2 to detect over-expressed Smad2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of Smad2 in lysates of COS transfected with Smad2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGF?. Low levels of staining in control lanes correspond to detection of endogenous Smad2. Preincubation of the antibody with immunizing peptide (data not shown) completely blocks specific band staining. The blot presented is askew relative to the molecular weight markers. The expected MW for Smad2 is 52 kDa. The membrane was probed with the primary antibody at a 1:2500 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.
- Validation comment
- Western Blot