Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX23447 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX23447, RRID:AB_385053
- Product name
- NFATC4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of NFAT3 in 25ug of various whole cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with NFAT3 antibody at a dilution of 1:1000 overnight at 4¢XC on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a proper secondary. Membranes were washed and chemiluminescent detection performed.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NFAT3 in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with NFAT3 antibody at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with a proper secondary antibody . Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of NFAT3 from MCF7 cell lysate. The antigen-antibody complex was formed by incubating 500£gg whole cell lysate with 3£gg of NFAT3 antibody overnight on a rocking platform at 4¢XC. The immune-complex was captured on 50£gl Protein A/G Agarose. Captured immune-complexes were washed and eluted. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were washed in TBS-0.1%Tween 20 and probed with NFAT3 antibody and a proper secondary antibody. Membranes were washed and chemiluminescent detection performed.