600-401-676

antibody from Rockland Immunochemicals, Inc.

Targeting: WRNIP1 bA420G6.2, CFAP93, FAP93, FLJ22526, WHIP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-676

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-676, RRID:AB_2288536

Product name

Anti-Human WHIP (RABBIT) Antibody - 600-401-676

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot analysis is shown using Rockland's Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate.  ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected.  The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein.  See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) diluted 1:5,000 for 45 min.  IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot