PA1-41506

antibody from Invitrogen Antibodies

Targeting: SMAD7 MADH7, MADH8

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41506

Provider

Invitrogen Antibodies

Product name

SMAD7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with chimpanzee, canine, pufferfish, xenopus and zebrafish based on sequence homology. Suggested positive control: A549, HepG2.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Genome-Wide Analysis of Smad7-Mediated Transcription in Mouse Embryonic Stem Cells.

Meng G, Lauria A, Maldotti M, Anselmi F, Polignano IL, Rapelli S, Donna D, Oliviero S
International journal of molecular sciences 2021 Dec 18;22(24)

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miR-21 mediates nickel nanoparticle-induced pulmonary injury and fibrosis.

Mo Y, Zhang Y, Wan R, Jiang M, Xu Y, Zhang Q
Nanotoxicology 2020 Nov;14(9):1175-1197

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Protective effect of curcumin against ultraviolet A irradiation‑induced photoaging in human dermal fibroblasts.

Liu X, Zhang R, Shi H, Li X, Li Y, Taha A, Xu C
Molecular medicine reports 2018 May;17(5):7227-7237

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Matrine suppresses cardiac fibrosis by inhibiting the TGF‑β/Smad pathway in experimental diabetic cardiomyopathy.

Zhang Y, Cui L, Guan G, Wang J, Qiu C, Yang T, Guo Y, Liu Z
Molecular medicine reports 2018 Jan;17(1):1775-1781

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Spatial regulation of bone morphogenetic proteins (BMPs) in postnatal articular and growth plate cartilage.

Garrison P, Yue S, Hanson J, Baron J, Lui JC
PloS one 2017;12(5):e0176752

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NIM811 downregulates transforming growth factor‑β signal transduction in vivo and in vitro.

Chen J, Liu DG, Wang H, Wu XN, Cong M, You H, Jia JD
Molecular medicine reports 2016 Jan;13(1):522-8

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Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats.

Beaudoin MS, Snook LA, Arkell AM, Stefanson A, Wan Z, Simpson JA, Holloway GP, Wright DC
Obesity (Silver Spring, Md.) 2014 Jul;22(7):1632-42

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Smad7 regulates terminal maturation of chondrocytes in the growth plate.

Estrada KD, Wang W, Retting KN, Chien CT, Elkhoury FF, Heuchel R, Lyons KM
Developmental biology 2013 Oct 15;382(2):375-84

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SMAD7 in formalin fixed paraffin-embedded (FFPE) human liver. Samples were incubated in SMAD7 polyclonal antibody (Product # PA1-41506). Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz. Image using the standard format of this product.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. Effects of Cur on the expression of inflammation and collagen degradation-associated proteins in UVA-irradiated human dermal fibroblasts. (A) Western blot analysis was performed to investigate the protein expression of NF-kappaB, MMP-1 and MMP-3 using specific antibodies. (B) Relative expression of NF-kappaB, MMP-1 and MMP-3 proteins was quantified by densitometry. (C) Western blot analysis was performed to determine the protein expression of TGF-beta, Smad2/3 and Smad7 using specific antibodies. (D) Relative expression of TGF-beta, Smad2/3 and Smad7 proteins was quantified by densitometry. All data are presented as the mean +- standard deviation, n=3. **P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 6 Immunohistochemistry for SMADs in articular and growth plate cartilage. Formalin fixed, decalcified sections of 1-wk old mouse tibial cartilage were stained with anti-pSmad1/5/8 and anti-Smad7 antibody. Signals were developed with DAB substrate (brown color) with no counterstaining. pSmad1/5/8 represents activation of BMP intracellular signaling, while Smad7 is an I-SMAD that functions as a functional antagonist of BMP signaling. SZ, superficial zone; MZ, middle zone; DZ, deep zone; RZ, resting zone; PZ, proliferative zone; HZ, hypertrophic zone.