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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-25912 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAD7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a Smad7 polyclonal antibody (Product # PA5-25912) in ZR-75-1 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using a Smad7 polyclonal antibody (Product # PA5-25912). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a Smad7 polyclonal antibody (Product # PA5-25912) (1:25, 1 hr at 37°C). Primary antibody detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) (1:400, 50 min at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded lung carcinoma using a Smad7 polyclonal antibody (Product # PA5-25912), followed by HRP-conjugated secondary antibody and DAB staining.
