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- Antigen structure
- References [23]
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- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- MAB2029 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Smad7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat Smad7.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O15105- Isotype
- IgG
- Antibody clone number
- 293039
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Exosomes derived from human umbilical cord mesenchymal stem cells improve myocardial repair via upregulation of Smad7.
MicroRNA-21 Knockout Exacerbates Angiotensin II-Induced Thoracic Aortic Aneurysm and Dissection in Mice With Abnormal Transforming Growth Factor-β-SMAD3 Signaling.
Smooth muscle cells differentiated from mesenchymal stem cells are regulated by microRNAs and suitable for vascular tissue grafts.
High SMAD7 and p-SMAD2,3 expression is associated with environmental enteropathy in children.
Loss of KLF4 and consequential downregulation of Smad7 exacerbate oncogenic TGF-β signaling in and promote progression of hepatocellular carcinoma.
MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells.
Smad7 knockdown activates protein kinase RNA-associated eIF2α pathway leading to colon cancer cell death.
Hepatocyte-specific Smad7 deletion accelerates DEN-induced HCC via activation of STAT3 signaling in mice.
TGF-β Induces Endometriotic Progression via a Noncanonical, KLF11-Mediated Mechanism.
CUG-binding protein 1 regulates HSC activation and liver fibrogenesis.
The TGFβ-SMAD3 pathway inhibits IL-1α induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration.
Downregulation of the TGFβ Pseudoreceptor BAMBI in Non-Small Cell Lung Cancer Enhances TGFβ Signaling and Invasion.
MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis.
Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5.
microRNA-181a has a critical role in ovarian cancer progression through the regulation of the epithelial-mesenchymal transition.
Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy.
Angiotensin II upregulates Kv1.5 expression through ROS-dependent transforming growth factor-beta1 and extracellular signal-regulated kinase 1/2 signalings in neonatal rat atrial myocytes.
Loss of N-Myc interactor promotes epithelial-mesenchymal transition by activation of TGF-β/SMAD signaling.
Protons sensitize epithelial cells to mesenchymal transition.
The TGF-β/Smad repressor TG-interacting factor 1 (TGIF1) plays a role in radiation-induced intestinal injury independently of a Smad signaling pathway.
The integrative analysis of microRNA and mRNA expression in mouse uterus under delayed implantation and activation.
Nuclear function of Smad7 promotes myogenesis.
3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins.
Wang XL, Zhao YY, Sun L, Shi Y, Li ZQ, Zhao XD, Xu CG, Ji HG, Wang M, Xu WR, Zhu W
International journal of molecular medicine 2018 May;41(5):3063-3072
International journal of molecular medicine 2018 May;41(5):3063-3072
MicroRNA-21 Knockout Exacerbates Angiotensin II-Induced Thoracic Aortic Aneurysm and Dissection in Mice With Abnormal Transforming Growth Factor-β-SMAD3 Signaling.
Huang X, Yue Z, Wu J, Chen J, Wang S, Wu J, Ren L, Zhang A, Deng P, Wang K, Wu C, Ding X, Ye P, Xia J
Arteriosclerosis, thrombosis, and vascular biology 2018 May;38(5):1086-1101
Arteriosclerosis, thrombosis, and vascular biology 2018 May;38(5):1086-1101
Smooth muscle cells differentiated from mesenchymal stem cells are regulated by microRNAs and suitable for vascular tissue grafts.
Gu W, Hong X, Le Bras A, Nowak WN, Issa Bhaloo S, Deng J, Xie Y, Hu Y, Ruan XZ, Xu Q
The Journal of biological chemistry 2018 May 25;293(21):8089-8102
The Journal of biological chemistry 2018 May 25;293(21):8089-8102
High SMAD7 and p-SMAD2,3 expression is associated with environmental enteropathy in children.
Syed S, Dinallo V, Iqbal NT, Di Iorio L, Di Fusco D, Guleria S, Amadi BC, Sadiq K, Moskaluk C, Ali SA, Kelly P, Monteleone G
PLoS neglected tropical diseases 2018 Feb;12(2):e0006224
PLoS neglected tropical diseases 2018 Feb;12(2):e0006224
Loss of KLF4 and consequential downregulation of Smad7 exacerbate oncogenic TGF-β signaling in and promote progression of hepatocellular carcinoma.
Sun H, Peng Z, Tang H, Xie D, Jia Z, Zhong L, Zhao S, Ma Z, Gao Y, Zeng L, Luo R, Xie K
Oncogene 2017 May 25;36(21):2957-2968
Oncogene 2017 May 25;36(21):2957-2968
MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells.
Li CX, Talele NP, Boo S, Koehler A, Knee-Walden E, Balestrini JL, Speight P, Kapus A, Hinz B
Nature materials 2017 Mar;16(3):379-389
Nature materials 2017 Mar;16(3):379-389
Smad7 knockdown activates protein kinase RNA-associated eIF2α pathway leading to colon cancer cell death.
De Simone V, Bevivino G, Sedda S, Izzo R, Laudisi F, Dinallo V, Franzè E, Colantoni A, Ortenzi A, Salvatori S, Rossi P, Sica GS, Fantini MC, Stolfi C, Monteleone G
Cell death & disease 2017 Mar 16;8(3):e2681
Cell death & disease 2017 Mar 16;8(3):e2681
Hepatocyte-specific Smad7 deletion accelerates DEN-induced HCC via activation of STAT3 signaling in mice.
Feng T, Dzieran J, Yuan X, Dropmann A, Maass T, Teufel A, Marhenke S, Gaiser T, Rückert F, Kleiter I, Kanzler S, Ebert MP, Vogel A, Ten Dijke P, Dooley S, Meindl-Beinker NM
Oncogenesis 2017 Jan 30;6(1):e294
Oncogenesis 2017 Jan 30;6(1):e294
TGF-β Induces Endometriotic Progression via a Noncanonical, KLF11-Mediated Mechanism.
Correa LF, Zheng Y, Delaney AA, Khan Z, Shenoy CC, Daftary GS
Endocrinology 2016 Sep;157(9):3332-43
Endocrinology 2016 Sep;157(9):3332-43
CUG-binding protein 1 regulates HSC activation and liver fibrogenesis.
Wu X, Wu X, Ma Y, Shao F, Tan Y, Tan T, Gu L, Zhou Y, Sun B, Sun Y, Wu X, Xu Q
Nature communications 2016 Nov 17;7:13498
Nature communications 2016 Nov 17;7:13498
The TGFβ-SMAD3 pathway inhibits IL-1α induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration.
Tjomsland V, Sandnes D, Pomianowska E, Cizmovic ST, Aasrum M, Brusevold IJ, Christoffersen T, Gladhaug IP
Journal of experimental & clinical cancer research : CR 2016 Jul 29;35(1):122
Journal of experimental & clinical cancer research : CR 2016 Jul 29;35(1):122
Downregulation of the TGFβ Pseudoreceptor BAMBI in Non-Small Cell Lung Cancer Enhances TGFβ Signaling and Invasion.
Marwitz S, Depner S, Dvornikov D, Merkle R, Szczygieł M, Müller-Decker K, Lucarelli P, Wäsch M, Mairbäurl H, Rabe KF, Kugler C, Vollmer E, Reck M, Scheufele S, Kröger M, Ammerpohl O, Siebert R, Goldmann T, Klingmüller U
Cancer research 2016 Jul 1;76(13):3785-801
Cancer research 2016 Jul 1;76(13):3785-801
MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis.
Murugaiyan G, da Cunha AP, Ajay AK, Joller N, Garo LP, Kumaradevan S, Yosef N, Vaidya VS, Weiner HL
The Journal of clinical investigation 2015 Mar 2;125(3):1069-80
The Journal of clinical investigation 2015 Mar 2;125(3):1069-80
Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5.
Bardita C, Predescu DN, Sha F, Patel M, Balaji G, Predescu SA
Journal of cell science 2015 Apr 15;128(8):1528-41
Journal of cell science 2015 Apr 15;128(8):1528-41
microRNA-181a has a critical role in ovarian cancer progression through the regulation of the epithelial-mesenchymal transition.
Parikh A, Lee C, Joseph P, Marchini S, Baccarini A, Kolev V, Romualdi C, Fruscio R, Shah H, Wang F, Mullokandov G, Fishman D, D'Incalci M, Rahaman J, Kalir T, Redline RW, Brown BD, Narla G, DiFeo A
Nature communications 2014;5:2977
Nature communications 2014;5:2977
Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy.
Mehuron T, Kumar A, Duarte L, Yamauchi J, Accorsi A, Girgenrath M
Skeletal muscle 2014;4:14
Skeletal muscle 2014;4:14
Angiotensin II upregulates Kv1.5 expression through ROS-dependent transforming growth factor-beta1 and extracellular signal-regulated kinase 1/2 signalings in neonatal rat atrial myocytes.
Lu G, Xu S, Peng L, Huang Z, Wang Y, Gao X
Biochemical and biophysical research communications 2014 Nov 21;454(3):410-6
Biochemical and biophysical research communications 2014 Nov 21;454(3):410-6
Loss of N-Myc interactor promotes epithelial-mesenchymal transition by activation of TGF-β/SMAD signaling.
Devine DJ, Rostas JW, Metge BJ, Das S, Mulekar MS, Tucker JA, Grizzle WE, Buchsbaum DJ, Shevde LA, Samant RS
Oncogene 2014 May 15;33(20):2620-8
Oncogene 2014 May 15;33(20):2620-8
Protons sensitize epithelial cells to mesenchymal transition.
Wang M, Hada M, Saha J, Sridharan DM, Pluth JM, Cucinotta FA
PloS one 2012;7(7):e41249
PloS one 2012;7(7):e41249
The TGF-β/Smad repressor TG-interacting factor 1 (TGIF1) plays a role in radiation-induced intestinal injury independently of a Smad signaling pathway.
Hneino M, François A, Buard V, Tarlet G, Abderrahmani R, Blirando K, Hoodless PA, Benderitter M, Milliat F
PloS one 2012;7(5):e35672
PloS one 2012;7(5):e35672
The integrative analysis of microRNA and mRNA expression in mouse uterus under delayed implantation and activation.
Su RW, Lei W, Liu JL, Zhang ZR, Jia B, Feng XH, Ren G, Hu SJ, Yang ZM
PloS one 2010 Nov 29;5(11):e15513
PloS one 2010 Nov 29;5(11):e15513
Nuclear function of Smad7 promotes myogenesis.
Miyake T, Alli NS, McDermott JC
Molecular and cellular biology 2010 Feb;30(3):722-35
Molecular and cellular biology 2010 Feb;30(3):722-35
3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins.
Seong HA, Jung H, Kim KT, Ha H
The Journal of biological chemistry 2007 Apr 20;282(16):12272-89
The Journal of biological chemistry 2007 Apr 20;282(16):12272-89
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Smad7 by Western Blot. Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Smad7 by Simple WesternTM. Simple Western lane view shows lysates of Sf 21 S. frugiperda insect ovarian cell line transfected with human Smad7, loaded at 0.2 mg/mL. A specific band was detected for Smad7 at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Smad7 in Human Liver. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Smad7 in Human Liver Cancer Tissue. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
