Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-82232 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FHL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RFDCHHCNES LFGKKYILRE ESPYCVVCFE TLFANTCEEC GKPIGCDCKD LSYKDRHWHE ACFHCSQCRN SLVDKPFAAK EDQLLCTDCY SNEYSSKCQE CKKTIMPGTR KMEYKGSS
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FHL2 by a FHL2 polyclonal antibody (Product # PA5-82232). Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FHL2 by a FHL2 polyclonal antibody (Product # PA5-82232). Analysis in human cell line U-2 OS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FHL2 in U-2 OS cells using a FHL2 polyclonal antibody (Product # PA5-82232). The analysis shows localization to actin filaments & focal adhesion sites.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FHL2 in human heart muscle and cerebral cortex tissues using a FHL2 polyclonal antibody (Product # PA5-82232). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FHL2 in human heart muscle using a FHL2 polyclonal antibody (Product # PA5-82232). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FHL2 in human cerebral cortex using a FHL2 polyclonal antibody (Product # PA5-82232). The analysis shows low expression as expected.