PA5-52234

antibody from Invitrogen Antibodies

Targeting: LRP2 DBS, gp330

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52234

Provider

Invitrogen Antibodies

Product name

LRP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GFTSMSDRPG KRCAAEGSSP LLLLPDNVRI RKYNLSSERF SEYLQDEEYI QAVDYDWDPE DIGLSVVYYT VRGEGSRFGA IKRAYIPNFE SGRNNLVQEV DLKLKYVMQP DGIAVDWVGR HIYWSDVKNK RIEVAKLDGR YRKWLISTDL

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-LRP2 Polyclonal Antibody (Product # PA5-52234) and a 522 kDa band corresponding to LRP2 was observed in Mouse Kidney and Rat Kidney which are reported to be the highly expressed tissue models. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4), Mouse Lung (Lane 5), Rat Lung (Lane 6), Mouse Kidney (Lane 7) and Rat Kidney (Lane 8) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of LRP2 in human kidney and skeletal muscle tissues using LRP2 Polyclonal Antibody (Product # PA5-52234). Corresponding LRP2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of LRP2 in human kidney using LRP2 Polyclonal Antibody (Product # PA5-52234) shows strong cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of LRP2 in human thyroid gland using LRP2 Polyclonal Antibody (Product # PA5-52234) shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of LRP2 in human cerebral cortex using LRP2 Polyclonal Antibody (Product # PA5-52234) shows moderate cytoplasmic positivity in neuronal cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of LRP2 in human skeletal muscle using LRP2 Polyclonal Antibody (Product # PA5-52234) shows no positivity in myocytes as expected.