Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-52234 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GFTSMSDRPG KRCAAEGSSP LLLLPDNVRI RKYNLSSERF SEYLQDEEYI QAVDYDWDPE DIGLSVVYYT VRGEGSRFGA IKRAYIPNFE SGRNNLVQEV DLKLKYVMQP DGIAVDWVGR HIYWSDVKNK RIEVAKLDGR YRKWLISTDL Highest antigen sequence identity to the following orthologs: Mouse - 78%, Rat - 78%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-LRP2 Polyclonal Antibody (Product # PA5-52234) and a 522 kDa band corresponding to LRP2 was observed in Mouse Kidney and Rat Kidney which are reported to be the highly expressed tissue models. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4), Mouse Lung (Lane 5), Rat Lung (Lane 6), Mouse Kidney (Lane 7) and Rat Kidney (Lane 8) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LRP2 in human kidney and skeletal muscle tissues using LRP2 Polyclonal Antibody (Product # PA5-52234). Corresponding LRP2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LRP2 in human kidney using LRP2 Polyclonal Antibody (Product # PA5-52234) shows strong cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LRP2 in human thyroid gland using LRP2 Polyclonal Antibody (Product # PA5-52234) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LRP2 in human cerebral cortex using LRP2 Polyclonal Antibody (Product # PA5-52234) shows moderate cytoplasmic positivity in neuronal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LRP2 in human skeletal muscle using LRP2 Polyclonal Antibody (Product # PA5-52234) shows no positivity in myocytes as expected.