PA5-22102

antibody from Invitrogen Antibodies

Targeting: GLDC GCSP, NKH

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-22102 - Provider product page
Provider: Invitrogen Antibodies
Product name: GLDC Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HePG2. Predicted reactivity: Mouse (91%), Rat (84%), Xenopus laevis (86%), Chicken (85%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.46 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GLDC protein structure - PA5-22102 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of glycine dehydrogenase (decarboxylating) precursor showing staining in the mitochondria of HepG2 cells. HepG2 cells were fixed in ice-cold MeOH for 5 min and stained using a glycine dehydrogenase (decarboxylating) precursor polyclonal antibody (Product # PA5-22102) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of GLDC was performed in Hep G2 cells fixed in ice-cold MeOH for 5 min. Green: GLDC Polyclonal Antibody (Product # PA5-22102) diluted at 1:400. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of GLDC was performed using 70% confluent log phase HeLa cells. The cells were fixed with ice-cold acetone for 5 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GLDC Polyclonal Antibody (Product # PA5-22102) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green).Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.