Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-83374 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83374, RRID:AB_11042818
- Product name
- Rabbit Polyclonal GLDC Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat GLDC antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance.
Kim D, Fiske BP, Birsoy K, Freinkman E, Kami K, Possemato RL, Chudnovsky Y, Pacold ME, Chen WW, Cantor JR, Shelton LM, Gui DY, Kwon M, Ramkissoon SH, Ligon KL, Kang SW, Snuderl M, Vander Heiden MG, Sabatini DM
Nature 2015 Apr 16;520(7547):363-7
Nature 2015 Apr 16;520(7547):363-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GLDC Antibody [NBP1-83374] - Western blot analysis in mouse liver tissue and rat liver tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GLDC Antibody [NBP1-83374] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-GLDC antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GLDC Antibody [NBP1-83374] - Western blot analysis in human cell lines Caco-2 and HeLa. Corresponding GLDC RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human kidney shows moderate granular positivity in cytoplasm in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Analysis in human placenta and skeletal muscle tissues. Corresponding GLDC RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human liver shows strong granular positivity in cytoplasm in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-83374] - Staining of human skeletal muscle shows no positivity in myocytes as expected.