Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-26504 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLDC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a GLDC polyclonal antibody (Product # PA5-26504) in mouse liver tissue lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Caco-2 (Lane 2), tissue extracts of Mouse Liver (Lane 3) and Rat Liver (Lane 4). The blot was probed with Anti-GLDC Polyclonal Antibody (Product # PA5-26504, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 112 kDa band corresponding to GLDC was observed across the cell lines and tissues tested. A non specific band at ~30 kDa was observed in cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Knockdown of GLDC was achieved by transfecting Hep G2 with GLDC specific siRNAs (Silencer® select Product # s223750, s5805). Western blot analysis (Fig. a) was performed using whole cell extracts from the GLDC knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GLDC Polyclonal Antibody (Product # PA5-26504, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GLDC.