Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- ELISA [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B5374 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ ACTN4 Antibody (clone 4D10) LS-B5374
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D10
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in PC-12.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ACTN4 monoclonal antibody (M01), clone 4D10 Western blot of ACTN4 expression in HeLa NE.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in Raw 264.7.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in MCF-7.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in NIH/3T3.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ACTN4 is approximately 0.1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ACTN4 is approximately 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-ACTN4 / Alpha-Actinin-4 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.