LS-C186290

antibody from LSBio

Targeting: ACTN4 FSGS1

Western blot (Enhanced validation)

Immunocytochemistry (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C186290

Provider

LSBio

Product name

ACTN4 Antibody (aa206-542) LS-C186290

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: A431 , B: Hep G2 . 7.5% SDS PAGE. ACTN4 antibody diluted at 1:1000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. ACTN4 antibody diluted at 1:1000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. ACTN4 antibody diluted at 1:1000.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5% SDS-PAGE. alpha Actinin 4 antibody [N2C1], Internal dilution:1:3000.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed HeLa using alpha Actinin 4 antibody at 1:200 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed HeLa using alpha Actinin 4 antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded A549 xenograft using alpha Actinin 4 antibody at 1:500 dilution.