Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-15357 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Alpha Actinin 4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract7.5 % SDS-PAGE dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - Alpha Actinin 4 antibody detects ACTN4 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract. 7.5% SDS-PAGE. Alpha Actinin 4 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - Paraffin-embedded mouse intestine alpha Actinin 4 antibody, C-term dilution: 1:500.