19096-1-AP

antibody from Proteintech Group

Targeting: ACTN4 FSGS1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

19096-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#19096-1-AP, RRID:AB_10642150

Product name

ACTN4 antibody

Antibody type

Polyclonal

Description

KD/KO validated ACTN4 antibody (Cat. #19096-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Disulfidptosis-Related Genes as Novel Biomarkers and Therapeutic Targets in Dilated Cardiomyopathy.

Bo X, Fan J, Xu S, Zeng Y, Xu A, Wang X, Wang H, Kang P, Fu Y, Fan H, Tan X, Zhou Y
Journal of inflammation research 2025;18:9967-9988

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RNF186 controls glucose metabolism in metabolic dysfunction-associated fatty liver disease.

Du J, Du Q, Zhang Y, Gao J, Wang C, Kou B, Jiang Y, Zhao D, Lin J
Cell & bioscience 2025 Nov 28;15(1):164

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DANCR knockdown alleviates neuroinflammation and functional recovery after spinal cord injury via regulating the ACTN4 / STAT3 axis.

Xia B, Yu C, Liu J, Lin J, Lyu J, Wang X, Zhu L
Archives of biochemistry and biophysics 2025 Mar;765:110293

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High MICAL-L2 promotes cancer progression and drug resistance in renal clear cell carcinoma cells through stabilization of ACTN4 following vimentin expression.

Zhao W, Qi C, Mao Y, Ye F, Xia T, Zhao M, Min P, Zhang Y, Du J
Biochimica et biophysica acta. Molecular basis of disease 2025 Mar;1871(3):167628

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Characterization of ACTN4 as a novel antiviral target against SARS-CoV-2.

Zhu M, Huang F, Sun H, Liu K, Chen Z, Yu B, Hao H, Liu H, Ding S, Zhang X, Liu L, Zhang K, Ren J, Liu Y, Liu H, Shan C, Guan W
Signal transduction and targeted therapy 2024 Sep 18;9(1):243

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Integrative Omics Uncovers Low Tumorous Magnesium Content as A Driver Factor of Colorectal Cancer.

Zhang R, Hu M, Liu Y, Li W, Xu Z, He S, Lu Y, Gong Y, Wang X, Hai S, Li S, Qi S, Li Y, Shu Y, Du D, Zhang H, Xu H, Zhou Z, Lei P, Chen HN, Dai L
Genomics, proteomics & bioinformatics 2024 Oct 15;22(4)

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AGR2-mediated unconventional secretion of 14-3-3ε and α-actinin-4, responsive to ER stress and autophagy, drives chemotaxis in canine mammary tumor cells.

Yuan SH, Wu CC, Wang YC, Chan XY, Chu HW, Yang Y, Liu HP
Cellular & molecular biology letters 2024 May 31;29(1):84

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KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinoma.

Yang Z, Peng Y, Wang Y, Yang P, Huang Z, Quan T, Xu X, Sun P, Sun Y, Lv J, Wei D, Zhou GQ
Oncogene 2024 Jun;43(23):1779-1795

---

Borg5 restricts contractility and motility in epithelial MDCK cells.

Cohen D, Fernandez D, Lázaro-Diéguez F, Überheide B, Müsch A
Journal of cell science 2024 Dec 1;137(23)

---

CCT3/ACTN4/TFRC axis protects hepatocellular carcinoma cells from ferroptosis by inhibiting iron endocytosis.

Zhu H, Liu Q, Meng Q, Zhang L, Ju S, Lang J, Zhu D, Chen Y, Aishan N, Ouyang X, Zhang S, Jin L, Xiao L, Wang L, Li L, Ji F
Journal of experimental & clinical cancer research : CR 2024 Aug 29;43(1):245

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Stool Protein Mass Spectrometry Identifies Biomarkers for Early Detection of Diffuse-type Gastric Cancer.

Ho CC, Gilbert MB, Urtecho G, Lee H, Drew DA, Klempner SJ, Cho JS, Ryan TJ, Rustgi N, Lee H, Lee J, Caraballo A, Magicheva-Gupta MV, Rios C, Shin AE, Tseng YY, Davis JL, Chung DC, Chan AT, Wang HH, Ryeom S
Cancer prevention research (Philadelphia, Pa.) 2024 Aug 1;17(8):361-376

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Dysregulation of the progranulin-driven autophagy-lysosomal pathway mediates secretion of the nuclear protein TDP-43.

Tanaka Y, Ito SI, Honma Y, Hasegawa M, Kametani F, Suzuki G, Kozuma L, Takeya K, Eto M
The Journal of biological chemistry 2023 Nov;299(11):105272

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GIPC3 couples to MYO6 and PDZ domain proteins, and shapes the hair cell apical region.

Chatterjee P, Morgan CP, Krey JF, Benson C, Goldsmith J, Bateschell M, Ricci AJ, Barr-Gillespie PG
Journal of cell science 2023 May 15;136(10)

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GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region.

Chatterjee P, Morgan CP, Krey JF, Benson C, Goldsmith J, Bateschell M, Ricci AJ, Barr-Gillespie PG
bioRxiv : the preprint server for biology 2023 Mar 1;

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Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.

Chen X, Du Z, Huang M, Wang D, Fong WP, Liang J, Fan L, Wang Y, Yang H, Chen Z, Hu M, Xu R, Li Y
Cancer immunology, immunotherapy : CII 2022 Mar;71(3):661-674

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Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.

Peng W, Liu Y, Qi H, Li Q
Reproductive biology and endocrinology : RB&E 2021 Mar 23;19(1):48

---

Protein tyrosine phosphatase 1B(PTP1B) promotes melanoma cells progression through Src activation.

Wang Q, Pan Y, Zhao L, Qi F, Liu J
Bioengineered 2021 Dec;12(1):8396-8406

---

The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.

Kollimada S, Senger F, Vignaud T, Théry M, Blanchoin L, Kurzawa L
Molecular biology of the cell 2021 Aug 19;32(18):1737-1748

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Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.

Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G
Journal of extracellular vesicles 2020;9(1):1716513

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NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.

Chen W, Wang W, Sun X, Xie S, Xu X, Liu M, Yang C, Li M, Zhang W, Liu W, Wang L, Zhou T, Yang Y
Cell death & disease 2020 Jul 14;11(7):534

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Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

Peng W, Tong C, Li L, Huang C, Ran Y, Chen X, Bai Y, Liu Y, Zhao J, Tan B, Luo X, Wang H, Wen L, Zhang C, Zhang H, Ding Y, Qi H, Baker PN
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6327-6338

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Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.

Zhong J, Lan C, Zhang C, Yang Y, Chen WX, Zhang KY, Zhao HL, Fang XY, Li HH, Tan L, Wang P, Ge HF, Hu R, Feng H
Journal of cellular biochemistry 2019 Jul;120(7):11008-11021

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Transactivated Epidermal Growth Factor Receptor Recruitment of α-actinin-4 From F-actin Contributes to Invasion of Brain Microvascular Endothelial Cells by Meningitic Escherichia coli.

Fu J, Li L, Yang X, Yang R, Amjad N, Liu L, Tan C, Chen H, Wang X
Frontiers in cellular and infection microbiology 2018;8:448

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Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.

Ge H, Yu A, Chen J, Yuan J, Yin Y, Duanmu W, Tan L, Yang Y, Lan C, Chen W, Feng H, Hu R
Scientific reports 2016 Nov 22;6:37681

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Alpha-actinin 4 is associated with cancer cell motility and is a potential biomarker in non-small cell lung cancer.

Wang MC, Chang YH, Wu CC, Tyan YC, Chang HC, Goan YG, Lai WW, Cheng PN, Liao PC
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2015 Feb;10(2):286-301

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α-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.

Hodges JL, Vilchez SM, Asmussen H, Whitmore LA, Horwitz AR
PloS one 2014;9(7):e101770

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Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.

Bai C, Tang S, Bai C, Chen X
Amino acids 2014 Apr;46(4):841-52

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MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.

Agarwal N, Adhikari AS, Iyer SV, Hekmatdoost K, Welch DR, Iwakuma T
Oncogene 2013 Jan 24;32(4):462-70

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 19096-1-AP(ACTN4 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using ACTN4 antibody 19096-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ACTN4 (IP:19096-1-AP, 5ug; Detection:19096-1-AP 1:1000) with HeLa cells lysate 3000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 19096-1-AP(ACTN4 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 19096-1-AP(ACTN4 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue

Flow cytometry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The ACTN4 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human ACTN4. This antibody recognizes human,mouse,rat antigen. The ACTN4 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP, FC analysis.

Appendix

19096-1-AP.pdf

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