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- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 19096-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#19096-1-AP, RRID:AB_10642150
- Product name
- ACTN4 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ACTN4 antibody (Cat. #19096-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Dysregulation of the progranulin-driven autophagy-lysosomal pathway mediates secretion of the nuclear protein TDP-43.
GIPC3 couples to MYO6 and PDZ domain proteins, and shapes the hair cell apical region.
GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region.
Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.
Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.
Protein tyrosine phosphatase 1B(PTP1B) promotes melanoma cells progression through Src activation.
The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.
NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.
Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.
Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.
Transactivated Epidermal Growth Factor Receptor Recruitment of α-actinin-4 From F-actin Contributes to Invasion of Brain Microvascular Endothelial Cells by Meningitic Escherichia coli.
Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.
Alpha-actinin 4 is associated with cancer cell motility and is a potential biomarker in non-small cell lung cancer.
α-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.
Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.
MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.
Tanaka Y, Ito SI, Honma Y, Hasegawa M, Kametani F, Suzuki G, Kozuma L, Takeya K, Eto M
The Journal of biological chemistry 2023 Nov;299(11):105272
The Journal of biological chemistry 2023 Nov;299(11):105272
GIPC3 couples to MYO6 and PDZ domain proteins, and shapes the hair cell apical region.
Chatterjee P, Morgan CP, Krey JF, Benson C, Goldsmith J, Bateschell M, Ricci AJ, Barr-Gillespie PG
Journal of cell science 2023 May 15;136(10)
Journal of cell science 2023 May 15;136(10)
GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region.
Chatterjee P, Morgan CP, Krey JF, Benson C, Goldsmith J, Bateschell M, Ricci AJ, Barr-Gillespie PG
bioRxiv : the preprint server for biology 2023 Mar 1;
bioRxiv : the preprint server for biology 2023 Mar 1;
Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.
Chen X, Du Z, Huang M, Wang D, Fong WP, Liang J, Fan L, Wang Y, Yang H, Chen Z, Hu M, Xu R, Li Y
Cancer immunology, immunotherapy : CII 2022 Mar;71(3):661-674
Cancer immunology, immunotherapy : CII 2022 Mar;71(3):661-674
Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.
Peng W, Liu Y, Qi H, Li Q
Reproductive biology and endocrinology : RB&E 2021 Mar 23;19(1):48
Reproductive biology and endocrinology : RB&E 2021 Mar 23;19(1):48
Protein tyrosine phosphatase 1B(PTP1B) promotes melanoma cells progression through Src activation.
Wang Q, Pan Y, Zhao L, Qi F, Liu J
Bioengineered 2021 Dec;12(1):8396-8406
Bioengineered 2021 Dec;12(1):8396-8406
The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.
Kollimada S, Senger F, Vignaud T, Théry M, Blanchoin L, Kurzawa L
Molecular biology of the cell 2021 Aug 19;32(18):1737-1748
Molecular biology of the cell 2021 Aug 19;32(18):1737-1748
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.
Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G
Journal of extracellular vesicles 2020;9(1):1716513
Journal of extracellular vesicles 2020;9(1):1716513
NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.
Chen W, Wang W, Sun X, Xie S, Xu X, Liu M, Yang C, Li M, Zhang W, Liu W, Wang L, Zhou T, Yang Y
Cell death & disease 2020 Jul 14;11(7):534
Cell death & disease 2020 Jul 14;11(7):534
Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.
Peng W, Tong C, Li L, Huang C, Ran Y, Chen X, Bai Y, Liu Y, Zhao J, Tan B, Luo X, Wang H, Wen L, Zhang C, Zhang H, Ding Y, Qi H, Baker PN
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6327-6338
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6327-6338
Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.
Zhong J, Lan C, Zhang C, Yang Y, Chen WX, Zhang KY, Zhao HL, Fang XY, Li HH, Tan L, Wang P, Ge HF, Hu R, Feng H
Journal of cellular biochemistry 2019 Jul;120(7):11008-11021
Journal of cellular biochemistry 2019 Jul;120(7):11008-11021
Transactivated Epidermal Growth Factor Receptor Recruitment of α-actinin-4 From F-actin Contributes to Invasion of Brain Microvascular Endothelial Cells by Meningitic Escherichia coli.
Fu J, Li L, Yang X, Yang R, Amjad N, Liu L, Tan C, Chen H, Wang X
Frontiers in cellular and infection microbiology 2018;8:448
Frontiers in cellular and infection microbiology 2018;8:448
Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.
Ge H, Yu A, Chen J, Yuan J, Yin Y, Duanmu W, Tan L, Yang Y, Lan C, Chen W, Feng H, Hu R
Scientific reports 2016 Nov 22;6:37681
Scientific reports 2016 Nov 22;6:37681
Alpha-actinin 4 is associated with cancer cell motility and is a potential biomarker in non-small cell lung cancer.
Wang MC, Chang YH, Wu CC, Tyan YC, Chang HC, Goan YG, Lai WW, Cheng PN, Liao PC
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2015 Feb;10(2):286-301
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2015 Feb;10(2):286-301
α-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.
Hodges JL, Vilchez SM, Asmussen H, Whitmore LA, Horwitz AR
PloS one 2014;9(7):e101770
PloS one 2014;9(7):e101770
Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.
Bai C, Tang S, Bai C, Chen X
Amino acids 2014 Apr;46(4):841-52
Amino acids 2014 Apr;46(4):841-52
MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.
Agarwal N, Adhikari AS, Iyer SV, Hekmatdoost K, Welch DR, Iwakuma T
Oncogene 2013 Jan 24;32(4):462-70
Oncogene 2013 Jan 24;32(4):462-70
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 19096-1-AP(ACTN4 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using ACTN4 antibody 19096-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-ACTN4 (IP:19096-1-AP, 5ug; Detection:19096-1-AP 1:1000) with HeLa cells lysate 3000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 19096-1-AP(ACTN4 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 19096-1-AP(ACTN4 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ACTN4 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human ACTN4. This antibody recognizes human,mouse,rat antigen. The ACTN4 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP, FC analysis.
