GTX113115

antibody from GeneTex

Targeting: ACTN4 FSGS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX113115

Provider

GeneTex

Proper citation

GeneTex Cat#GTX113115, RRID:AB_1949579

Product name

alpha Actinin 4 antibody [N2C1], Internal

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX113115 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by western blot analysis. A. 30 ?g PC-12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [N2C1], Internal (GTX113115) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) B: HepG2 (GTX27900) 7.5% SDS PAGE GTX113115 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX113115 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX113115) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX113115) antibody at 1:500 dilution.