Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX113115 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113115, RRID:AB_1949579
- Product name
- alpha Actinin 4 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX113115 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- alpha Actinin 4 antibody [N2C1], Internal detects alpha Actinin 4 protein by western blot analysis. A. 30 ?g PC-12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [N2C1], Internal (GTX113115) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) B: HepG2 (GTX27900) 7.5% SDS PAGE GTX113115 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX113115 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX113115) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX113115) antibody at 1:500 dilution.