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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX113116 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113116, RRID:AB_11173447
- Product name
- alpha Actinin 4 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE GTX113116 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE GTX113116 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Actinin 4 antibody [N1N2], N-term detects ACTN4 protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract7.5 % SDS-PAGEalpha Actinin 4 antibody [N1N2], N-term (GTX113116) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Actinin 4 antibody detects alpha Actinin 4 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody (GTX113116) diluted by 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Actinin 4 antibody [N1N2], N-term detects ACTN4 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: ACTN4 protein stained by alpha Actinin 4 antibody [N1N2], N-term (GTX113116) diluted at 1:500.Blue: Hoechst 33342 staining.
