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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA346384 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-ACTN4 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-ACTN4 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ACTN4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Lanes: Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate; Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate; Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate; Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 5000; Gene Name: ACTN4; Submitted by: Johannes W. Hell, University of California;
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB Suggested Anti-ACTN4 Antibody Titration: 0.625 ug/ml; Positive Control: HCT116 cell lysate; ACTN4 is supported by BioGPS gene expression data to be expressed in HCT116
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample Type: ACTNX-GFP transfected COS-7 cells Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit-Alexa-Fluor 568; Secondary Antibody: ilution: 1: 100; Color/Signal Descriptions: Green: GFP Red: ACTN4; Gene Name: ACTN4; Submitted by: Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710
- Validation comment
- IHC
