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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27475 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Actinin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, mouse brain, PC-12.
- Concentration
- 0.32 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 4 Polyclonal Antibody detects ACTN4 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw264.7 whole cell lysate/extract. G. 30 µg C2C12 whole cell lysate/extract.7.5% SDS-PAGE. Alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 5% SDS PAGE. alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 4 Polyclonal Antibody detects ACTN4 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract. E. 30 µg A375 whole cell lysate/extract.7.5% SDS-PAGE. Alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 4 Polyclonal Antibody detects ACTN4 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw264.7 whole cell lysate/extract. G. 30 µg C2C12 whole cell lysate/extract.7.5% SDS-PAGE. Alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 4 Polyclonal Antibody detects ACTN4 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.7.5% SDS-PAGE. Alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475) and a ~110 kDa band corresponding to Alpha-actinin-4 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), A-431 (Lane 3), IMR-90 (Lane 4), PANC-1 (Lane 5), DU 145 (Lane 6), LNCaP (Lane 7), PC-12 (Lane 8), NIH/3T3 (Lane 9), Mouse Kidney (Lane 10), Rat Kidney (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).https://doi.org/10.1016/j.bbrc.2019.12.084
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha Actinin 4 in paraformaldehyde-fixed HeLa cells using an Alpha Actinin 4 polyclonal antibody (Product # PA5-27475) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 4 Polyclonal Antibody detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 (Product # PA5-27475) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
