PA5-27475

FSGS1

Antibody data

Submit

Validation data

Product number: PA5-27475
Provider: Invitrogen Antibodies
Product name: Anti-alpha Actinin 4 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-27475
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: PA5-27475 targets alpha Actinin 4 in IF/ICC, IHC (P), and WB applications and shows reactivity with Human, Rat and mouse samples. The PA5-27475 immunogen is recombinant fragment corresponding to a region within amino acids 698 and 911 of Human alpha Actinin 4. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.32 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 4 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate (D) 30 µg HepG2 whole cell lysate and E) 30 µg A375 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Alpha Actinin 4 polyclonal antibody (Product # PA5-27475) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 4 using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw264.7 whole cell lysate and G) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Alpha Actinin 4 polyclonal antibody (Product # PA5-27475) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 4 using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an Alpha Actinin 4 polyclonal antibody (Product # PA5-27475) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 4 using 30 µg PC-12 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Alpha Actinin 4 polyclonal antibody (Product # PA5-27475) at a dilution of 1:1000.