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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27475 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Actinin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, mouse brain, PC-12. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (85%), Xenopus laevis (92%), Chicken (94%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.32 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha Actinin 4 in paraformaldehyde-fixed HeLa cells using an Alpha Actinin 4 polyclonal antibody (Product # PA5-27475) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 4 Polyclonal Antibody detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alpha Actinin 4 Polyclonal Antibody (Product # PA5-27475) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 (Product # PA5-27475) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
