Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- 12058-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12058-1-AP, RRID:AB_2156630
- Product name
- ODF2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ODF2 antibody (Cat. #12058-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole.
Analyzing Centrioles and Cilia by Expansion Microscopy.
Cilium induction triggers differentiation of glioma stem cells.
Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development.
ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
The essential role of intraflagellar transport protein IFT81 in male mice spermiogenesis and fertility.
Abnormal fertility, acrosome formation, IFT20 expression and localization in conditional Gmap210 knockout mice.
A DNAH17 missense variant causes flagella destabilization and asthenozoospermia.
Centrosome anchoring regulates progenitor properties and cortical formation.
Murine germ cell-specific disruption of Ift172 causes defects in spermiogenesis and male fertility.
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
Chromosome misalignment is associated with PLK1 activity at cenexin-positive mitotic centrosomes.
Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice†.
Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.
Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry.
Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
Asymmetric Centriole Numbers at Spindle Poles Cause Chromosome Missegregation in Cancer.
Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice.
Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa.
A mitotic kinase scaffold depleted in testicular seminomas impacts spindle orientation in germ line stem cells.
Myosin Id is required for planar cell polarity in ciliated tracheal and ependymal epithelial cells.
Developmental alterations in centrosome integrity contribute to the post-mitotic state of mammalian cardiomyocytes.
Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles.
Structurally distinct Ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility.
Rab11 endosomes contribute to mitotic spindle organization and orientation.
Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules.
Shen XL, Yuan JF, Qin XH, Song GP, Hu HB, Tu HQ, Song ZQ, Li PY, Xu YL, Li S, Jian XX, Li JN, He CY, Yu XP, Liang LY, Wu M, Han QY, Wang K, Li AL, Zhou T, Zhang YC, Wang N, Li HY
The Journal of cell biology 2022 Jan 3;221(1)
The Journal of cell biology 2022 Jan 3;221(1)
Analyzing Centrioles and Cilia by Expansion Microscopy.
Kong D, Loncarek J
Methods in molecular biology (Clifton, N.J.) 2021;2329:249-263
Methods in molecular biology (Clifton, N.J.) 2021;2329:249-263
Cilium induction triggers differentiation of glioma stem cells.
Goranci-Buzhala G, Mariappan A, Ricci-Vitiani L, Josipovic N, Pacioni S, Gottardo M, Ptok J, Schaal H, Callaini G, Rajalingam K, Dynlacht B, Hadian K, Papantonis A, Pallini R, Gopalakrishnan J
Cell reports 2021 Sep 7;36(10):109656
Cell reports 2021 Sep 7;36(10):109656
Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development.
Xiao C, Grzonka M, Meyer-Gerards C, Mack M, Figge R, Bazzi H
EMBO reports 2021 Feb 3;22(2):e51127
EMBO reports 2021 Feb 3;22(2):e51127
ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
Ran J, Liu M, Feng J, Li H, Ma H, Song T, Cao Y, Zhou P, Wu Y, Yang Y, Yang Y, Yu F, Guo H, Zhang L, Xie S, Li D, Gao J, Zhang X, Zhu X, Zhou J
Developmental cell 2020 May 4;53(3):287-299.e5
Developmental cell 2020 May 4;53(3):287-299.e5
The essential role of intraflagellar transport protein IFT81 in male mice spermiogenesis and fertility.
Qu W, Yuan S, Quan C, Huang Q, Zhou Q, Yap Y, Shi L, Zhang D, Guest T, Li W, Yee SP, Zhang L, Cazin C, Hess RA, Ray PF, Kherraf ZE, Zhang Z
American journal of physiology. Cell physiology 2020 Jun 1;318(6):C1092-C1106
American journal of physiology. Cell physiology 2020 Jun 1;318(6):C1092-C1106
Abnormal fertility, acrosome formation, IFT20 expression and localization in conditional Gmap210 knockout mice.
Wang Z, Shi Y, Ma S, Huang Q, Yap YT, Shi L, Zhang S, Zhou T, Li W, Hu B, Zhang L, Krawetz SA, Pazour GJ, Hess RA, Zhang Z
American journal of physiology. Cell physiology 2020 Jan 1;318(1):C174-C190
American journal of physiology. Cell physiology 2020 Jan 1;318(1):C174-C190
A DNAH17 missense variant causes flagella destabilization and asthenozoospermia.
Zhang B, Ma H, Khan T, Ma A, Li T, Zhang H, Gao J, Zhou J, Li Y, Yu C, Bao J, Ali A, Murtaza G, Yin H, Gao Q, Jiang X, Zhang F, Liu C, Khan I, Zubair M, Hussain HMJ, Khan R, Yousaf A, Yuan L, Lu Y, Xu X, Wang Y, Tao Q, Hao Q, Fang H, Cheng H, Zhang Y, Shi Q
The Journal of experimental medicine 2020 Feb 3;217(2)
The Journal of experimental medicine 2020 Feb 3;217(2)
Centrosome anchoring regulates progenitor properties and cortical formation.
Shao W, Yang J, He M, Yu XY, Lee CH, Yang Z, Joyner AL, Anderson KV, Zhang J, Tsou MB, Shi H, Shi SH
Nature 2020 Apr;580(7801):106-112
Nature 2020 Apr;580(7801):106-112
Murine germ cell-specific disruption of Ift172 causes defects in spermiogenesis and male fertility.
Zhang S, Liu Y, Huang Q, Yuan S, Liu H, Shi L, Yap YT, Li W, Zhen J, Zhang L, Hess RA, Zhang Z
Reproduction (Cambridge, England) 2020 Apr;159(4):409-421
Reproduction (Cambridge, England) 2020 Apr;159(4):409-421
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.
Bowler M, Kong D, Sun S, Nanjundappa R, Evans L, Farmer V, Holland A, Mahjoub MR, Sui H, Loncarek J
Nature communications 2019 Mar 1;10(1):993
Nature communications 2019 Mar 1;10(1):993
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
So C, Seres KB, Steyer AM, Mönnich E, Clift D, Pejkovska A, Möbius W, Schuh M
Science (New York, N.Y.) 2019 Jun 28;364(6447)
Science (New York, N.Y.) 2019 Jun 28;364(6447)
Chromosome misalignment is associated with PLK1 activity at cenexin-positive mitotic centrosomes.
Colicino EG, Stevens K, Curtis E, Rathbun L, Bates M, Manikas J, Amack J, Freshour J, Hehnly H
Molecular biology of the cell 2019 Jun 15;30(13):1598-1609
Molecular biology of the cell 2019 Jun 15;30(13):1598-1609
Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice†.
Shi L, Zhou T, Huang Q, Zhang S, Li W, Zhang L, Hess RA, Pazour GJ, Zhang Z
Biology of reproduction 2019 Jul 1;101(1):188-199
Biology of reproduction 2019 Jul 1;101(1):188-199
Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.
Shen Y, Zhang F, Li F, Jiang X, Yang Y, Li X, Li W, Wang X, Cheng J, Liu M, Zhang X, Yuan G, Pei X, Cai K, Hu F, Sun J, Yan L, Tang L, Jiang C, Tu W, Xu J, Wu H, Kong W, Li S, Wang K, Sheng K, Zhao X, Yue H, Yang X, Xu W
Nature communications 2019 Jan 25;10(1):433
Nature communications 2019 Jan 25;10(1):433
Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry.
Bärenz F, Kschonsak YT, Meyer A, Jafarpour A, Lorenz H, Hoffmann I
Molecular biology of the cell 2018 Dec 15;29(26):3105-3118
Molecular biology of the cell 2018 Dec 15;29(26):3105-3118
Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
Tu HQ, Qin XH, Liu ZB, Song ZQ, Hu HB, Zhang YC, Chang Y, Wu M, Huang Y, Bai YF, Wang G, Han QY, Li AL, Zhou T, Liu F, Zhang XM, Li HY
Nature communications 2018 Dec 11;9(1):5277
Nature communications 2018 Dec 11;9(1):5277
Asymmetric Centriole Numbers at Spindle Poles Cause Chromosome Missegregation in Cancer.
Cosenza MR, Cazzola A, Rossberg A, Schieber NL, Konotop G, Bausch E, Slynko A, Holland-Letz T, Raab MS, Dubash T, Glimm H, Poppelreuther S, Herold-Mende C, Schwab Y, Krämer A
Cell reports 2017 Aug 22;20(8):1906-1920
Cell reports 2017 Aug 22;20(8):1906-1920
Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice.
Zhang Z, Li W, Zhang Y, Zhang L, Teves ME, Liu H, Strauss JF 3rd, Pazour GJ, Foster JA, Hess RA, Zhang Z
Molecular biology of the cell 2016 Sep 28;
Molecular biology of the cell 2016 Sep 28;
Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa.
Dai J, Xu W, Zhao X, Zhang M, Zhang D, Nie D, Bao M, Wang Z, Wang L, Qiao Z
Reproduction (Cambridge, England) 2016 Mar;151(3):227-37
Reproduction (Cambridge, England) 2016 Mar;151(3):227-37
A mitotic kinase scaffold depleted in testicular seminomas impacts spindle orientation in germ line stem cells.
Hehnly H, Canton D, Bucko P, Langeberg LK, Ogier L, Gelman I, Santana LF, Wordeman L, Scott JD
eLife 2015 Sep 25;4:e09384
eLife 2015 Sep 25;4:e09384
Myosin Id is required for planar cell polarity in ciliated tracheal and ependymal epithelial cells.
Hegan PS, Ostertag E, Geurts AM, Mooseker MS
Cytoskeleton (Hoboken, N.J.) 2015 Oct;72(10):503-16
Cytoskeleton (Hoboken, N.J.) 2015 Oct;72(10):503-16
Developmental alterations in centrosome integrity contribute to the post-mitotic state of mammalian cardiomyocytes.
Zebrowski DC, Vergarajauregui S, Wu CC, Piatkowski T, Becker R, Leone M, Hirth S, Ricciardi F, Falk N, Giessl A, Just S, Braun T, Weidinger G, Engel FB
eLife 2015 Aug 6;4
eLife 2015 Aug 6;4
Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles.
Kong D, Farmer V, Shukla A, James J, Gruskin R, Kiriyama S, Loncarek J
The Journal of cell biology 2014 Sep 29;206(7):855-65
The Journal of cell biology 2014 Sep 29;206(7):855-65
Structurally distinct Ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility.
Chung JJ, Shim SH, Everley RA, Gygi SP, Zhuang X, Clapham DE
Cell 2014 May 8;157(4):808-22
Cell 2014 May 8;157(4):808-22
Rab11 endosomes contribute to mitotic spindle organization and orientation.
Hehnly H, Doxsey S
Developmental cell 2014 Mar 10;28(5):497-507
Developmental cell 2014 Mar 10;28(5):497-507
Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules.
Wu JM, Chen CT, Coumar MS, Lin WH, Chen ZJ, Hsu JT, Peng YH, Shiao HY, Lin WH, Chu CY, Wu JS, Lin CT, Chen CP, Hsueh CC, Chang KY, Kao LP, Huang CY, Chao YS, Wu SY, Hsieh HP, Chi YH
Proceedings of the National Academy of Sciences of the United States of America 2013 May 7;110(19):E1779-87
Proceedings of the National Academy of Sciences of the United States of America 2013 May 7;110(19):E1779-87
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse testis tissue were subjected to SDS PAGE followed by western blot with 12058-1-AP(ODF2 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-ODF2 (IP:12058-1-AP, 5ug; Detection:12058-1-AP 1:500) with mouse testis tissue lysate 8000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ODF2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ODF2. This antibody recognizes human,mouse,rat antigen. The ODF2 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.