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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V2600 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Perlecan Antibody (HSPG)
- Antibody type
- Monoclonal
- Antigen
- A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Bovine, Porcine, Simian
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- A7L6
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Perlecan antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Breeding Strategy Determines Rupture Incidence in Post-Infarct Healing WARPing Cardiovascular Research.
Proteoglycans in Normal and Healing Skin.
Fractone aging in the subventricular zone of the lateral ventricle.
X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function.
Perlecan deficiency causes endothelial dysfunction by reducing the expression of endothelial nitric oxide synthase.
Perlecan is required for FGF-2 signaling in the neural stem cell niche.
Confocal microscopy demonstrates association of LTBP-2 in fibrillin-1 microfibrils and colocalisation with perlecan in the disc cell pericellular matrix.
Matrilysin/matrix metalloproteinase-7(MMP7) cleavage of perlecan/HSPG2 creates a molecular switch to alter prostate cancer cell behavior.
Transcriptional activation by NFκB increases perlecan/HSPG2 expression in the desmoplastic prostate tumor microenvironment.
The role of vascular-derived perlecan in modulating cell adhesion, proliferation and growth factor signaling.
Deckx S, Carai P, Bateman J, Heymans S, Papageorgiou AP
PloS one 2015;10(9):e0139199
PloS one 2015;10(9):e0139199
Proteoglycans in Normal and Healing Skin.
Smith MM, Melrose J
Advances in wound care 2015 Mar 1;4(3):152-173
Advances in wound care 2015 Mar 1;4(3):152-173
Fractone aging in the subventricular zone of the lateral ventricle.
Kerever A, Yamada T, Suzuki Y, Mercier F, Arikawa-Hirasawa E
Journal of chemical neuroanatomy 2015 Jul-Sep;66-67:52-60
Journal of chemical neuroanatomy 2015 Jul-Sep;66-67:52-60
X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function.
Ceko MJ, Hummitzsch K, Hatzirodos N, Bonner WM, Aitken JB, Russell DL, Lane M, Rodgers RJ, Harris HH
Metallomics : integrated biometal science 2015 Jan;7(1):71-82
Metallomics : integrated biometal science 2015 Jan;7(1):71-82
Perlecan deficiency causes endothelial dysfunction by reducing the expression of endothelial nitric oxide synthase.
Nonaka R, Iesaki T, de Vega S, Daida H, Okada T, Sasaki T, Arikawa-Hirasawa E
Physiological reports 2015 Jan 1;3(1)
Physiological reports 2015 Jan 1;3(1)
Perlecan is required for FGF-2 signaling in the neural stem cell niche.
Kerever A, Mercier F, Nonaka R, de Vega S, Oda Y, Zalc B, Okada Y, Hattori N, Yamada Y, Arikawa-Hirasawa E
Stem cell research 2014 Mar;12(2):492-505
Stem cell research 2014 Mar;12(2):492-505
Confocal microscopy demonstrates association of LTBP-2 in fibrillin-1 microfibrils and colocalisation with perlecan in the disc cell pericellular matrix.
Hayes AJ, Gibson MA, Shu C, Melrose J
Tissue & cell 2014 Jun;46(3):185-97
Tissue & cell 2014 Jun;46(3):185-97
Matrilysin/matrix metalloproteinase-7(MMP7) cleavage of perlecan/HSPG2 creates a molecular switch to alter prostate cancer cell behavior.
Grindel BJ, Martinez JR, Pennington CL, Muldoon M, Stave J, Chung LW, Farach-Carson MC
Matrix biology : journal of the International Society for Matrix Biology 2014 Jun;36:64-76
Matrix biology : journal of the International Society for Matrix Biology 2014 Jun;36:64-76
Transcriptional activation by NFκB increases perlecan/HSPG2 expression in the desmoplastic prostate tumor microenvironment.
Warren CR, Grindel BJ, Francis L, Carson DD, Farach-Carson MC
Journal of cellular biochemistry 2014 Jul;115(7):1322-33
Journal of cellular biochemistry 2014 Jul;115(7):1322-33
The role of vascular-derived perlecan in modulating cell adhesion, proliferation and growth factor signaling.
Lord MS, Chuang CY, Melrose J, Davies MJ, Iozzo RV, Whitelock JM
Matrix biology : journal of the International Society for Matrix Biology 2014 Apr;35:112-22
Matrix biology : journal of the International Society for Matrix Biology 2014 Apr;35:112-22
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human testicular carcinoma stained with Perlecan antibody (A7L6).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Perlecan Antibody (clone A7L6). Confirmation of Integrity and Purity of the Antibody.
