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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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- Product number
- MA5-14641 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Perlecan Monoclonal Antibody (A7L6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-14641 targets Perlecan in IF, IHC (F), and IHC (P, F) applications and shows reactivity with Bovine, Human, mouse, and Porcine samples.
- Antibody clone number
- A7L6
- Concentration
- 0.2 mg/mL
Submitted references Multiplex in situ hybridization within a single transcript: RNAscope reveals dystrophin mRNA dynamics.
Metalloprotease inhibitor TIMP proteins control FGF-2 bioavailability and regulate skeletal growth.
Mechanosensing by Peyer's patch stroma regulates lymphocyte migration and mucosal antibody responses.
(89)Zr-DFO-AMG102 Immuno-PET to Determine Local Hepatocyte Growth Factor Protein Levels in Tumors for Enhanced Patient Selection.
PDGFRβ regulates craniofacial development through homodimers and functional heterodimers with PDGFRα.
Liver aging and pseudocapillarization in a Werner syndrome mouse model.
LARGE glycans on dystroglycan function as a tunable matrix scaffold to prevent dystrophy.
Anti-DNA autoantibodies initiate experimental lupus nephritis by binding directly to the glomerular basement membrane in mice.
DOT1L regulates dystrophin expression and is critical for cardiac function.
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
Synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with Trichinella spiralis but is not essential for nurse cell development.
Heparan sulfate proteoglycans as regulators of fibroblast growth factor-2 signaling in brain endothelial cells. Specific role for glypican-1 in glioma angiogenesis.
Hildyard JCW, Rawson F, Wells DJ, Piercy RJ
PloS one 2020;15(9):e0239467
PloS one 2020;15(9):e0239467
Metalloprotease inhibitor TIMP proteins control FGF-2 bioavailability and regulate skeletal growth.
Saw S, Aiken A, Fang H, McKee TD, Bregant S, Sanchez O, Chen Y, Weiss A, Dickson BC, Czarny B, Sinha A, Fosang A, Dive V, Waterhouse PD, Kislinger T, Khokha R
The Journal of cell biology 2019 Sep 2;218(9):3134-3152
The Journal of cell biology 2019 Sep 2;218(9):3134-3152
Mechanosensing by Peyer's patch stroma regulates lymphocyte migration and mucosal antibody responses.
Chang JE, Buechler MB, Gressier E, Turley SJ, Carroll MC
Nature immunology 2019 Nov;20(11):1506-1516
Nature immunology 2019 Nov;20(11):1506-1516
(89)Zr-DFO-AMG102 Immuno-PET to Determine Local Hepatocyte Growth Factor Protein Levels in Tumors for Enhanced Patient Selection.
Price EW, Carnazza KE, Carlin SD, Cho A, Edwards KJ, Sevak KK, Glaser JM, de Stanchina E, Janjigian YY, Lewis JS
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2017 Sep;58(9):1386-1394
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2017 Sep;58(9):1386-1394
PDGFRβ regulates craniofacial development through homodimers and functional heterodimers with PDGFRα.
Fantauzzo KA, Soriano P
Genes & development 2016 Nov 1;30(21):2443-2458
Genes & development 2016 Nov 1;30(21):2443-2458
Liver aging and pseudocapillarization in a Werner syndrome mouse model.
Cogger VC, Svistounov D, Warren A, Zykova S, Melvin RG, Solon-Biet SM, O'Reilly JN, McMahon AC, Ballard JW, De Cabo R, Le Couteur DG, Lebel M
The journals of gerontology. Series A, Biological sciences and medical sciences 2014 Sep;69(9):1076-86
The journals of gerontology. Series A, Biological sciences and medical sciences 2014 Sep;69(9):1076-86
LARGE glycans on dystroglycan function as a tunable matrix scaffold to prevent dystrophy.
Goddeeris MM, Wu B, Venzke D, Yoshida-Moriguchi T, Saito F, Matsumura K, Moore SA, Campbell KP
Nature 2013 Nov 7;503(7474):136-40
Nature 2013 Nov 7;503(7474):136-40
Anti-DNA autoantibodies initiate experimental lupus nephritis by binding directly to the glomerular basement membrane in mice.
Krishnan MR, Wang C, Marion TN
Kidney international 2012 Jul;82(2):184-92
Kidney international 2012 Jul;82(2):184-92
DOT1L regulates dystrophin expression and is critical for cardiac function.
Nguyen AT, Xiao B, Neppl RL, Kallin EM, Li J, Chen T, Wang DZ, Xiao X, Zhang Y
Genes & development 2011 Feb 1;25(3):263-74
Genes & development 2011 Feb 1;25(3):263-74
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
Nigro J, White JF, Ramshaw JA, Haylock DN, Nilsson SK, Werkmeister JA
Biomaterials 2010 Jul;31(21):5689-99
Biomaterials 2010 Jul;31(21):5689-99
Synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with Trichinella spiralis but is not essential for nurse cell development.
Beiting DP, Park PW, Appleton JA
Infection and immunity 2006 Mar;74(3):1941-3
Infection and immunity 2006 Mar;74(3):1941-3
Heparan sulfate proteoglycans as regulators of fibroblast growth factor-2 signaling in brain endothelial cells. Specific role for glypican-1 in glioma angiogenesis.
Qiao D, Meyer K, Mundhenke C, Drew SA, Friedl A
The Journal of biological chemistry 2003 May 2;278(18):16045-53
The Journal of biological chemistry 2003 May 2;278(18):16045-53
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Frozen human tonsil stained with Heparan Sulfate Proteoglycan antibody using peroxidase-conjugate and DAB chromogen. Note basement membrane and cell surface staining of blood vessels.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Perlecan showing staining in the cytoplasm (Basement membrane cell) of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Perlecan Rat Monoclonal Antibody (Product # MA5-14641) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
