AF2404

antibody from Novus Biologicals

Targeting: CD38

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2404

Provider

Novus Biologicals

Product name

Sheep Polyclonal CD38 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human CD38 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse CD38 is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CD38 by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, RPMI 8226 human multiple myeloma cell line, and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human CD38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2404) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific and diffuse band was detected for CD38 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CD38 by Simple WesternTM. Detection of Human CD38 by Simple WesternTM.