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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ARG54960 - Provider product page
- Provider
- Arigo
- Product name
- anti-CD38 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 241-270 (C-terminus) of Human CD38.
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of RPMI8226 cell line stained with ARG54960 anti-CD38 antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 293T cell line stained with ARG54960 anti-CD38 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cell stained with ARG54960 anti-CD38 antibody (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human hepatocarcinoma tissue stained with ARG54960 anti-CD38 antibody (green) at 0.05 mg/ml dilution. Red counterstaining is PI.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded human hepatocarcinoma stained with ARG54960 anti-CD38 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HepG2 cells stained with ARG54960 anti-CD38 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
