Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [2]
 - Immunocytochemistry [2]
 - Immunohistochemistry [1]
 - Flow cytometry [1]
 
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Validation data
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- Product number
 - F48288 - Provider product page

 - Provider
 - NSJ Bioreagents
 - Product name
 - CD38 Antibody
 - Antibody type
 - Polyclonal
 - Description
 - This highly specific CD38 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human samples.
 - Reactivity
 - Human
 - Host
 - Rabbit
 - Conjugate
 - Unconjugated
 - Vial size
 - 0.08 ml, 0.4 ml
 - Concentration
 - In 1X PBS, pH 7.4, with 0.09% sodium azide
 - Storage
 - Aliquot the CD38 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
 
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					Supportive validation
					
									
				
				- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Western blot analysis of lysate from 293T cell line using CD38 antibody diluted at 1:1000.
 
- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Western blot analysis of lysate from RPMI8226 cell line using CD38 antibody diluted at 1:1000.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence analysis of CD38 antibody with paraffin-embedded human hepatocarcinoma tissue. Primary Ab was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. Red counterstaining is PI.
 
- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Confocal immunofluorescent analysis of CD38 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
 
							
					Supportive validation
					
									
				
				- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - IHC analysis of FFPE human hepatocarcinoma with CD38 antibody
 
							
					Supportive validation
					
									
				
		- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Flow cytometric analysis of HepG2 cells using CD38 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.