MA1-182

antibody from Invitrogen Antibodies

Targeting: CD38

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-182

Provider

Invitrogen Antibodies

Product name

CD38 Monoclonal Antibody (5C5C3)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

By Western blot, MA1-182 detects a band at ~45kD, which likely represents the glycosylated form of CD38. Different lysis buffers can be used to prepare lysates for Western blot with MA1-182, but depending on the level of CD38 expression, it may be necessary to use a membrane extraction buffer (e.g. Mem-PER Plus Membrane Protein Extraction Kit, Product # 89842).

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5C5C3

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD38 was performed by loading 50 µg of the indicated cell lysates prepared using IP Lysis Buffer (Product # 87788) or Mem-PER Plus Membrane Protein Extraction Kit (Product # 89842), and 10 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a nitrocellulose membrane (Product # 88014) using the Power Blotter (Product # 22834), and blocked with 5% milk in TBST for at least 1 hour at room temperature. CD38 was detected at ~45 kD using a CD38 monoclonal antibody (Product # MA1-182) at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:40,000 for at least 30 minutes. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD38 on Ramos cells. Cells were fixed, permeabilized, and stained with a CD38 monoclonal antibody (Product # MA1-182, blue histogram) or with a mouse IgG1 isotype control (Product # MA5-14453, red histogram) at a concentration of 1 µg/mL. After incubation with the primary antibody for 1 hour on ice, the cells were stained with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) for at least 30 minutes on ice. A representative 10,000 cells were acquired for each sample.